The Sekelsky Lab

pP{Target70}

pP{Target70} is a derivative of pP{TargetB}. The CaSpeR-derived mini-white gene was replaced by a white cDNA expressed under the basal hsp70 promoter, much like the pW8 vector or P{FRT-w^hs-FRT} construct.

pP{Target70} Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAG ctagagttgttggttggcacaccacaaatatactgttgccgagcacaattccgctcgac GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCggatccAGATCTGCTAGCGGCCGCA TGCCGCGGACGCGTAGGCCTGCAGGAATTCGATTCGACCTCGAGGGGGGGCCCGGTACC ACCAAACTGTCTCACGACGTTTTGcaattctagtatgtatgtaagttaataaaacccat ttttgcggaaagtagataaaaaaaacattttttttttttactgcactggatccttgccc cgttattctctattcgttttgtgactctccctctctgtactattgctctctcactctgt cgcacagtaaacggcactctattctcgttgcttcgagagagcgcgcctcgaatgttcgc gaaaagagcgccggagtataaatagaggcgcttcgtcgacggagcgtcaattcaattca aacaagCAAAGTGAACACATCGCGAAGCAAGCTGAGCAAACAAACAAGCGCAGCGAACA AGCTAAACAATCTGCAATAAAGTGCAAGTTAAAGTGAATCAATTAAAAGTAACCAACAA CCAAGTAATTAAACTAAAAACTGCAACTACTGAAATCAACCAAGAAGTCATTATTGAAG ACAAGAAGAGAACTCTgaataggtcgatagcgtcaattcggcacgaggtttcgtgacga agctccaagcggtttacgccatcaattaaacacaaagtgctgtgccaaaactcc tctcgcttcttatttttgtttgttttttgagtgattgggtgggtgattggttttgggtgggtaagc aggggaaagtgtgaaaaatcccggcaATGGGCCAAGAGGATCAGGAGCTATTAATTCGCGGAGGCA GCAAACACCCATCTGCCGAGCATCTGAACAATGGTGACAGCGGAGCGGCTTCGCAGAGCTGCATTA ACCAGGGCTTCGGGCAGGCCAAAAACTACGGCACGCTCCTGCCACCCAGTCCGCCGGAGGACTCCG GTTCAGGGAGCGGCCAACTAGCCGAGAACCTCACCTATGCCTGGCACAATATGGACATCTTTGGGG CGGTCAATCAGCCGGGCTCCGGATGGCGGCAGCTGGTCAACCGGACACGCGGACTATTCTGCAACG AGCGACACATACCGGCGCCCAGGAAACATTTGCTCAAGAACGTTTGCGGCGTGGCCTATCCGGGCG AACTTTTGGCCGTGATGGGCAGTTCCGGTGCCGGAAAGACGACCCTGCTGAATGCCCTTGCCTTTC GATCGCCGCAGGGCATCCAAGTATCGCCATCCGGGATGCGACTGCTCAATGGCCAACCTGTGGACG CCAAGGAGATGCAGGCCAGGTGCGCCTATGTCCAGCAGGATGACCTCTTTATCGGCTCCCTAACGG CCAGGGAACACCTGATTTTCCAAGCCATGGTGCGGATGCCACGACATCTGACCTATCGGCAGCGAG TGGCCCGCGTGGATCAGGTGATCCAGGAGCTTTCGCTCAGCAAATGTCAGCACACGATCATCGGTG TGCCCGGCAGGGTGAAAGGTCTGTCCGGCGGAGAAAGGAAGCGTCTGGCATTCGCCTCCGAGGCAC TAACCGATCCGCCGCTTCTGATCTGCGATGAGCCCACCTCCGGACTGGACTCATTTACCGCCCACA GCGTCGTCCAGGTGCTGAAGAAGCTGTCGCAGAAGGGCAAGACCGTCATCCTGACCATTCATCAGC CGTCTTCCGAGCTGTTTGAGCTCTTTGACAAGATCCTTCTGATGGCCGAGGGCAGGGTAGCTTTCT TGGGCACTCCCAGCGAAGCCGTCGACTTCTTTTCCTACGTGGGTGCCCAGTGTCCTACCAACTACA ATCCGGCGGACTTTTACGTACAGGTGTTGGCCGTTGTGCCCGGACGGGAGATCGAGTCCCGTGATC GGATCGCCAAGATATGCGACAATTTTGCCATTAGCAAAGTAGCCCGGGATATGGAGCAGTTGTTGG CCACCAAAAATCTGGAGAAGCCACTGGAGCAGCCGGAGAATGGGTACACCTACAAGGCCACCTGGT TCATGCAGTTCCGGGCGGTCCTGTGGCGATCCTGGCTGTCGGTGCTCAAGGAACCACTCCTCGTAA AAGTGCGACTTATTCAGACAACGATGGTTGCCATCTTGATTGGCCTCATCTTTTTGGGCCAACAAC TCACGCAAGTGGGTGTGATGAATATCAACGGAGCCATCTTCCTCTTCCTGACCAACATGACCTTTC AAAACGTCTTTGCCACGATAAATGTGTTCACCTCAGAGCTGCCAGTTTTTATGAGGGAGGCCCGAA GTCGACTTTATCGCTGTGACACATACTTTCTGGGCAAAACGATTGCCGAATTGCCGCTTTTTCTCA CAGTGCCACTGGTCTTCACGGCGATTGCCTATCCGATGATCGGACTGCGGGCCGGAGTGCTGCACT TCTTCAACTGCCTGGCGCTGGTCACTCTGGTGGCCAATGTGTCAACGTCCTTCGGATATCTAATAT CCTGCGCCAGCTCCTCGACCTCGATGGCGCTGTCTGTGGGTCCGCCGGTTATCATACCATTCCTGC TCTTTGGCGGCTTCTTCTTGAACTCGGGCTCGGTGCCAGTATACCTCAAATGGTTGTCGTACCTCT CATGGTTCCGTTACGCCAACGAGGGTCTGCTGATTAACCAATGGGCGGACGTGGAGCCGGGCGAAA TTAGCTGCACATCGTCGAACACCACGTGCCCCAGTTCGGGCAAGGTCATCCTGGAGACGCTTAACT TCTCCGCCGCCGATCTGCCGCTGGACTACGTGGGTCTGGCCATTCTCATCGTGAGCTTCCGGGTGC TCGCATATCTGGCTCTAAGACTTCGGGCCCGACGCAAGGAGTAGccgacatatatccgaaataact gcttgttttttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaa tgtaattatatttgtgccaataaaaacaagatatgacctatagaatacaaaaaaaaaaaaaaaaaa aaaaaaaaaaactcgatcgagtcgacGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCggatccaaacctagtaatta attcggctgctgctctAAACGACGCATTTCGTACTCCAAAGTACGAATTTTTTCCCTCA AGCTCTTATTTTCATTAAACAATGAACAGGACCTAACGCACAGTCACGTTATTGTTTAC ATAAATGATTTTTTTTACTATTCAAACTTACTCTGTTTGTGTACTCCCACTGGTATAGC CTTCTTTTATCTTTTCTGGTTCAGGCTCTATCACTTTACTAGGTACGGCATCTGCGTTG AGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATCATT AAAGTGGGTATCACAAATTTGGGAGTTTTCACCAAGGCTGCACCCAAGGCTCTGCTCCC ACAATTTTCTCTTAATAGCACACTTCGGCACGTGAATTAATTTTACTCCAGTCACAGCT TTGCAGCAAAATTTGCAATATTTCATTTTTTTTTATTCCACGTAAGGGTTAATGTTTTC AAAAAAAAATTCGTCCGCACACAACCTTTCCTCTCAACAAGCAAACGTGCACTGAATTT AAGTGTATACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTTATGTTATTTCATCAT Gggccagacccacgtagtccagcggcagatcggcggcggagaagttaagcgtctccagg atgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaatttcgcccgg ctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgaga ggtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaaggcgttt ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcg ctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcc actggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtg gtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagc cagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggt agcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaaga agatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag ggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaa tgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatg cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgct gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt gttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcgg ttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactc atggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttc tgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagtt gctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtg ctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgag atccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttca ccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagg gcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaa taggggttccgcgcacatttccccgaaaagtgccacctgcgaagtcttagagccagata
tgcgagcacccggaagctcacgatgagaatggccagac

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